Determination of Paeoniflorin in Qisheng Capsule
10.3870/yydb.2014.07.027
- VernacularTitle:芪生胶囊中芍药苷的含量测定
- Author:
Xiaopeng SHI
;
Shan MIAO
;
Linlin BI
;
Jie LI
;
Qing MIAO
;
Biyan DANG
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Qisheng capsule;
Paeoniflorin;
Content determination;
HPLC
- From:
Herald of Medicine
2014;(7):937-939
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a content determination method for paeoniflorin in qisheng capsule. Methods The quantitative analysis of paeoniflorin in qisheng Capsule was carried out by high-performance liquid chromatography ( HPLC) . The chromatographic separation was achieved by using a Kromasil C18 chromatographic column (4. 6 mm×250 mm,5 μm) with a mobile phase consisting of methanol,water (1585) at a flow rate of 1. 0 mL·min-1 and 230 nm detection wavelength. Results The linear range was 2. 5-12. 5 μg·mL-1( r =0. 999 9). The average recovery and RSD of the method were 99. 97%and 0. 94%. Conclusion The method is accurate,specific,reproducible,which can effectively be used in quality control of paeoniflorin in qisheng capsule.
