Study of bone marrow srt omal cells on a three-dimensional nano-zirconia porous scaffold for bone tissue engineering
- VernacularTitle:三维联通纳米氧化锆多孔支架对犬骨髓间充质干细胞附着生长的实验研究
- Author:
Yinglan ZHU
;
Ruiqiao ZHU
;
Xiaolei SHI
- Publication Type:Journal Article
- Keywords:
Bone tissue engineering;
Nano-ZrO2;
Bone marrow stomal cell
- From:
Journal of Medical Postgraduates
2014;(6):564-567
- CountryChina
- Language:Chinese
-
Abstract:
Objective Nowadays, in the field of bone tissue engineering , searching for a kind of scaffold , which is both strong enough and biocompatible , is still a hot topic .Objective of this study is to evaluate the three-dimensional nano-zirconia porous scaffold′s effect on bone marrow stromal cells early attachment and proliferation . Methods Preparing nano-zirconia porous scaffold and testing its porosity and its mechanical strength .Hemolysis experiment was taken to evaluate its biosafety .Canine bone marrow stromal cells ( BM-SCs) were extracted from an adult beagle and cultured in vitro.The third generation cells were seeded onto the scaffold and induced to os-teoblasts under condition medium .Cells early attachment on scaffold was observed by SEM .MTT and ALP tests were taken to evaluate cells proliferation and osteogenesis within two weeks . Results Nano-zirconia porous scaffold in our study presented a porous structure with a well connected inner .Its porosity and compressive strength were (92.667 ±0.324)%and 4.38 MPa, respectively.Hemolysis rate was 2.19%, which was demonstrated to be safe to human body .Canine BMSCs could colonize the surface of the materials , bridge macro-poros-ity and proliferate on the scaffold .A values taken by the method of MTT showed that cells could proliferate quickly on the scaffold after 3, 6, 9, 12 d (A values were 0.604 ±0.059, 1.215 ±0.019, 1.385 ± 0.254, and 1.506 ±0.050), comparing with that on the first day (A value was 0.261 ±0.034).Statistical difference was found (P <0.01) .The ALP level raised up significantly on day 7 and 14 ( A val-ues were 0.032 ±0.002 and 0.062 ±0.007), comparing with that on the first day (A value was 0.016 ±0.003).Statistical difference was found (P<0.01). Conclusion The three-dimensional nano-ZrO2 porous scaffold in our study presented satisfactory biocompati-bility.It could offer a microenvironment for osteoblasts growth and secreted osteogenesis marker in vitro .
