Expression and identification of recombinant protein of the new gene F10 which is associated with the path-ogenesis of hydatidiform mole
- VernacularTitle:葡萄胎病理相关新基因 F10重组蛋白的表达及鉴定
- Author:
Zhanjun PANG
;
Jungui ZHOU
- Publication Type:Journal Article
- Keywords:
F10 gene;
F10 recombinant protein;
Hydatidiform mole
- From:
Journal of Medical Postgraduates
2014;(6):577-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective F10 is a new gene cloned from hydatidiform moles .Our previous study showed that the F10 gene might be associated with the invasion of trophoblast cells .This study was to construct a prokaryotic plasmid expressing the recombinant protein of F10 and isolate, purify, and identify it in order to further investigate the function of the F10 gene. Methods The coding sequence of the F10 gene was amplified from the human tissue by RT-PCR and cloned into the vector pET 28a.The plasmid was ex-pressed in the E.coli BL21(DE3) to obtain the F10 recombinant protein.The expressed F10 recombinant protein was purified with the chromatographic column and verified by sodium dodecyl sulfate polyacrylamide gel electropheresis ( SDS-PAGE) . Results The pro-karyotic plasmid expressing the F 10 recombinant protein was successfully constructed and highly purified F 10 recombinant protein was obtained. Conclusion The F10 recombinant protein was expressed and highly purified , which could be used as an antigen for the preparation of the F10 monoclonal antibody .
