Effect of Rhodioloside on PI3-K/AKT signaling pathway on cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.1008-1372.2014.06.005
- VernacularTitle:红景天苷对大鼠脑缺血再灌注损伤后PI3-K／AKT 信号通路的影响
- Author:
Yinli LUO
;
Xiaosong HUANG
;
Lihong TAN
;
Chunyan YU
;
Liuqing LIU
- Publication Type:Journal Article
- Keywords:
RHODIOLA SACRA/pharmacology;
1-phosphatidylinositol 3-kinase;
p21-activated kinases;
Reperfusion injury;
Apoptosis
- From:
Journal of Chinese Physician
2014;(6):734-738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of protective effect of Rhodioloside in cerebral ischemia-reperfusion rats and its relevance to phosphatidylinositol 3-kinases ( PI3-K)/protein serine-threonine kinases ( AKT) signaling pathway .Methods Forty eight Sprague-Dawley rats were randomly divided into four groups: sham-operation group , ischemia-reperfusion group , and Rhodiolo-side treatment groups (5 and 10 mg/kg).The model of right middle cerebral artery occlusion was established with thread ligation meth -od.The score of the neurological deficit was estimated 2 h followed by 24 h reperfusion.Histopathological changes were observed by hematoxylin-eosin(HE) staining.The infarct volume was measured with triphenyltetrazolium chloride (TTC) staining.Apoptotic cells were assessed with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method.The expressions of PI3-K and p-AKT were evaluated with immunohistochemistry .Results The score of the neurological deficit was decreased more ob-viously, the number of apoptotic were decreased more significantly , the expressions of PI3-K and p-AKT were increased more signifi-cantly in the Rhodioloside treatment groups (5 and 10 mg/kg) than in the ischemia-reperfusion group ( P <0.05).The score of the neurological deficit was decreased , the number of apoptotic was decreased , and the expressions of PI 3-K and p-AKT were increased in the Rhodioloside treatment group (10 mg/kg) than the Rhodioloside treatment group (5 mg/kg) ( P <0.05).Conclusions The protective mechanism of Rhodioloside therapy against cerebral ischemia r-eperfusion injury might be associated with activating the PI 3-K/AKT signaling pathway and then inhibiting neuronal apoptosis .
