Relationships between the expressions of matrix metalloproteinase-9,Toll-like Receptor 4 and lung revascularization in pa-tients with chronic obstructive pulmonary disease
10.3760/cma.j.issn.1008-1372.2014.06.014
- VernacularTitle:TOLL 样受体4、基质金属蛋白酶-9在慢性阻塞性肺疾病肺血管中的表达及其与肺血管重建的关系
- Author:
Jinpeng CONG
;
Xinwen ZHANG
;
Jiaxing SUN
;
Lei DONG
;
Wenjia HAO
;
Wencheng YU
- Publication Type:Journal Article
- Keywords:
Toll-like receptor 4/metabolism;
Matrix metalloproteinase 9/metabolism;
Pulmonary disease,chronic obstructive
- From:
Journal of Chinese Physician
2014;(6):766-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between the expressions of matrix metalloproteinase -9 (MMP-9), Toll-likeReceptor 4 ( TLR4) and lung revascularization in patients with chronic obstructive pulmonary disease .Methods Lung tissues frompatients with chronic obstructive pulmonary disease (COPD) (COPD group,n =25) and those without COPD (non-COPD group,n =25) were obtained from surgically resected specimens .The ratio of the area of the wall to that of the pulmonary arterioles (WA %) andthe ratio of the thickness of the wall to the external diameter of the pulmonary arterioles (WT %) were analyzed by computer-based imageanalysis system.Immunohistochemical technique was applied to investigate the expressions of TLR 4, proliferative cell nuclear antigen(PCNA) and MMP-9 in vascular smooth muscle cells.Results ⑴ The inflammatory infiltration degree, WA %, and WT %were significantly higher than that of non -COPD group ( P <0.01), respectively.⑵Compared with non-COPD group, the expressionsof PCNA, TLR4, and MMP-9 in vascular smooth muscle cells were increased significantly ( P <0.01).⑶The expressions of TLR4,MMP-9 had a positive correlation with WA%, WT%, degree of inflammatory infiltration, and the expression of PCNA ( r =0.67,0.74,0.47,0.44;0.59,0.71,0.61,0.33, P <0.01), up-regulated expression of TLR4 was closely related with the expression of MMP-9 ( r =0.55, P <0.01).Conclusions The pulmonary arterioles of COPD patients showed marked inflammatory and arteriolemuscularization, the TLR4 might aggravate inflammation,induced upregulation of MMP-9 expression, played an important role in the pulmonary vascular remodeling process.
