Determination of Content and Encapsulation Efficiency of Genistein-loaded Eudragit Nanoparticles by HPLC
- VernacularTitle:HPLC法测定染料木素丙烯酸树脂纳米粒的药物含量及包封率
- Author:
Na XU
;
Hongyu JING
;
Jingling TANG
;
Linhua WU
- Publication Type:Journal Article
- Keywords:
Genistein;
Eudragit;
Nanoparticles;
HPLC;
Content;
Encapsulation efficiency
- From:
China Pharmacist
2014;(2):204-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods:The HPLC analysis was carried out on a DiamonsilTM C18(250 mm ×4.6 mm,5μm)column with the mobile phase of meth-anol-water (85∶15) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 261nm and the column temperature was 25℃. Results: A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1(r =0.999 8). The recovery of genistein was 99.85%(RSD =0. 65%,n=9). Conclusion:The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles.
