Optimization of Extraction for Water Soluble Component with Anti-hepatic Fibrosis Effects from Carthamus tinctorius L. via a Multi-index Orthogonal Method
10.3870/yydb.2014.07.025
- VernacularTitle:正交法优选红花抗肝纤维化的水溶性成分提取工艺
- Author:
Wenjing LI
;
Defu CAI
;
Qinglian YU
;
Yingcai NIU
;
Lina GUO
;
Bo HONG
- Publication Type:Journal Article
- Keywords:
Carthamus tinctorius L.;
Hydroxysafflor yellow A;
Extraction technology;
Orthogonal experiment;
Hepatic fibrosis
- From:
Herald of Medicine
2014;(7):931-934
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction process of anti-hepatic fibrosis water-soluble component from Carthamus tinctorius L. via the orthogonal method. Methods The influences of type and content of solvent,extraction time,and extraction frequency on processing were investigated by assessing the content of hydroxysafflor yellow A,yield of extraction and anti-hepatic fibrosis potency via the high performance liquid chromatography and enzyme-linked immunosorbent assay by L9(3)4 orthogonal test. Results The optimum extracting condition for the anti-hepatic fibrosis water-soluble component from carthamus tinctorius L. was as follows: extracting with 10 times the volume of 10% ethanol for twice, 0. 5 h in each. Conclusion The process is scientific and feasible, which serves as a guideline for the production of the anti-hepatic fibrosis water-soluble components.
