Comparison of two common insemination methods on in-vitro fertilization and embryo transfer
10.3969/j.issn.2095-4344.2014.27.023
- VernacularTitle:比较2种常用授精法在体外授精-胚胎移植中的差异
- Author:
Canqiao LUO
;
Yubin LI
;
Tao LI
;
Shan XIAO
;
Lingli LONG
;
Wenlin XIE
;
Zhaoming NIE
- Publication Type:Journal Article
- Keywords:
sperm;
fertilization,in vitro;
pregnancy rate
- From:
Chinese Journal of Tissue Engineering Research
2014;(27):4406-4411
- CountryChina
- Language:Chinese
-
Abstract:
The in-vitro fertilization and embryo transfer technique has been widely applied in human insemination. The rate of successful insemination is gradual y rising, and the in-vitro fertilization directly determine the insemination outcome.
OBJECTIVE:To evaluate the difference between the two common using insemination methods, microdrop and open, in in-vitro fertilization and embryo development.
METHODS:A randomized study was conducted to compare microdrop and open insemination methods among non-male factor patients undergoing in-vitro fertilization and embryo transfer. A total of 1 175 cases were enrol ed in the research. There were 573 cases in the microdrop group, and 602 cases in open insemination group. The fertilization rate and embryo development in the two groups were compared.
RESULTS AND CONCLUSION:The fertilization failure rate [total fertilization failure rate+low fertilization rate (<25%oocytes fertilized)] in the microdrop insemination group was higher than in the open insemination group (11.9%, 3.3%, P<0.001), while the good quality embryo rate and pregnancy rate did not differ significantly between the two groups (al P>0.05). The open insemination method is a simple insemination method with a lower fertilization failure rate. As the fertilization is a highly complicated process involving many extrinsic and intrinsic factors, further study is needed to confirm the effects of the two insemination methods on in-vitro fertilization outcome.
