Inhibition of PKD2 mediated apoptosis induced by 5, 7-dihydrox-8-nitrochrysin in U937 cell line
10.3760/cma.j.issn.1008-1372.2014.06.003
- VernacularTitle:抑制 PKD2介导8-硝基白杨素诱导 U937细胞凋亡
- Author:
Jianling YANG
;
Xiaozheng CAO
;
Lihua HE
;
Jianguo CAO
;
Jianfeng YANG
- Publication Type:Journal Article
- Keywords:
Protein kinases;
Plant extracts;
Leukemia,monocytic,acute;
Apoptosis
- From:
Journal of Chinese Physician
2014;(6):728-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether 5, 7-dihydrox-8-nitrochrysin (NOC) induces apoptosis in U937 monocytic leukae-mia cells is involved in the regulation of the activity of PKD 2.Methods U937 cell line cells were cultured in vitro .Apoptosis rate was analyzed by flow cytometry (FCM) using propidium iodide (PI) staining.DNA ladder bands were observed by DNA agarose gel electro-phoresis.The phosphorylated protein expression of PKD 2 was analyzed using Western blot .Results NOC (2.5, 5.0, and 10.0μmol/L) increased apoptosis rate in U937 cells in a concentration-dependent manner ( P <0.05).After treatment with NOC (5.0 and 10.0μmol/L) for 24 h, U937 cells presented typical DNA ladder bands .At the same time, not only did NOC effectively down-regulate the ex-pression of PDK2 phosphorylated protein , but also increased apoptosis rate in U 937 cells in the presence of G?6976, a specific inhibitor of PKD2.Conclusions The effect that NOC induces apoptosis in U 937 cells is related to the inhibition of the activity of PKD 2.
