Application of Fluorescence Quantitative PCR in Salmonella Rapid Detection in Drugs
- VernacularTitle:Taqman荧光定量PCR法在药品沙门菌快速检测中的应用
- Author:
Tingting LIU
;
Luhua WANG
;
Zunwen WANG
;
Shizhen ZHU
- Publication Type:Journal Article
- Keywords:
Salmonella;
Fluorescence quantitative PCR;
Taqman probe
- From:
China Pharmacist
2014;(2):242-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a Taqman fluorescence quantitative PCR for the detection of Salmonella in drugs. Methods:Based on Salmonella specific gene, a pair of primers and a probe were designed, and DNA of Salmonella was extracted for the detec-tion. Results:The method was much specific, and the detection limit was 160 cfu/ml. The detection rate was 100% for the artificially contaminated samples. Conclusion:Fluorescence quantitative PCR can be applied in the rapid detection of Salmonella in drugs.
