Determination of 4,5-Dicaffeoyquinic Acid in Elehantopus Scaber Linn by HPLC
- VernacularTitle:HPLC法测定地胆草中4,5-二咖啡酰奎宁酸的含量
- Author:
Yulei ZOU
;
Jialing GENG
- Publication Type:Journal Article
- Keywords:
Elehantopus scaber Linn;
4,5-Dicaffeoyquinic acid;
HPLC
- From:
China Pharmacist
2014;(2):312-314
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of 4,5-dicaffeoyquinic acid in Elehantopus scaber Linn. Methods:The separation was carried out on an Agilent SB C18 column(250 mm × 4. 6 mm, 5 μm). The mobile phase was acetoni-trile-0. 1% phosphoric acid (17∶83) and the flow rate was 1. 0 ml·min-1 . The detection wavelength was set at 327nm, the column temperature was 30℃ and the injection volume was 10 μl. Results: The calibration curve showed a good linearity over the range of 0. 023 5-2. 352 0 μg(r=0. 999 9). The average recovery was 98. 75% with RSD of 1. 24%(n=6). Conclusion: The method is simple, rapid and accurate, and can be used as a quantitative analysis method for Elehantopus scaber Linn.
