Determination of Salvianolic Acid B in Ansheng Yizhi Capsules by HPLC
- VernacularTitle:HPLC法测定安神益智胶囊中丹酚酸B的含量
- Author:
Huiping MA
;
Lanru LI
;
Zhicheng DONG
;
Lei HE
;
Zhengping JIA
- Publication Type:Journal Article
- Keywords:
HPLC;
Ansheng Yizhi capsules;
Salvianolic acid B;
Determination
- From:
China Pharmacist
2014;(2):314-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the content determination of salvianolic acid B in Ansheng Yizhi cap-sules. Methods:A Hypersil ODS2 C18 column(150 mm × 4. 6 mm,5 μm) was used and methanol-water-formic acid (40∶60∶1) was used as the mobile phase. The detection wavelength was at 286 nm. The flow rate was 1. 0 ml·min-1 and the sample size was 10 μl. Results:The calibration curve of salvianolic acid B was linear within the range 7. 75-77. 51 μg·ml-1(r=0. 999 6). The average re-covery was 98. 17%(RSD=1. 79%, n=6). Conclusion:The method is simple, accurate and repeatable, which can be used in the quality control of Ansheng Yizhi capsules.
