Determination of Triptolide in Compound Tripterygium Wilfordil Syrups by HPLC
- VernacularTitle:HPLC法测定复方雷公藤糖浆中雷公藤甲素的含量
- Author:
Xixia CHENG
;
Yunxia LI
- Publication Type:Journal Article
- Keywords:
Compound tripterygium wilfordil syrups;
Triptolide;
HPLC
- From:
China Pharmacist
2014;(2):316-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the content determination of triptolide in compound tripterygium wilfordil syrups by HPLC. Methods:The samples were analyzed on a Diamonsil C18 column(150 mm × 4. 6 mm,5μm), the mobile phase consisted of methanol and water (46∶54), the flow rate was 1. 0 ml·min-1, the detection wavelength was 224 nm, and the column temperature was kept at 30℃, and the injection volume was 10 μl. Results: The linear range of triptolide was 5. 66-33. 96 μg · ml-1 ( r =0. 999 8), and the average recovery was 99. 35% with RSD of 1. 02%(n=9). Conclusion:The method is simple and sensitive with good repeatability, and can be used to control the quality of triptolide in compound tripterygium wilfordil syrups.
