Effects of viola-containing serum on phagocytosis and TOLL-like receptor expression of macrophage
10.3969/j.issn.1000-484X.2014.06.009
- VernacularTitle:紫花地丁含药血清对巨噬细胞吞噬功能及TOLL样受体表达的影响
- Author:
Zhiwei ZHANG
;
Kun CAI
;
Mali WU
;
Honghong YU
;
Ling LENG
;
Wenpeng YUE
;
Weiyi TIAN
- Publication Type:Journal Article
- Keywords:
Viola;
Serum-pharmacological-method;
Macrophages;
TOLL-like-receptors
- From:
Chinese Journal of Immunology
2014;(6):759-762
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effects of viola on macrophage TOLL-like receptor expression ,and tentatively explore the partial mechanism of viola intervention on macrophage function.Methods: Using viola water decoction lavage intervention in clean grade SD rats of conventional preparation containing serum ,In a certain concentration coculture with mouse peritoneal macrophages in 96-well plates.After 24 hours,cell phagocytosis activity was determined by neutral red uptake assay ,changes in expression levels of TLR-1,TLR-2,TLR-3,TLR-4,TLR-5 mRNAs were determined by RT-PCR.Results:Compared with the same concentration of normal serum group :(1 ) viola-containing serum group macrophage activity was significantly increased ( P<0.01 );( 2 ) Within certain concentration,viola containing serum group with increased or decreased expression levels of TLR -1,TLR-2,TLR-3 mRNA(P<0.05 or P<0.01 ) ,the expression level of TLR-4 was not significantly altered in each group ( P>0.05 ) ,and the expression level of TLR-5 was significantly increased in each group ( P<0.01 ).Conclusion:Viola-containing serum has obvious promoting effect on macrophage phag-ocytosis,the mechanism of which may be related to that this drug regulates and controls part of macrophage TOLL -like receptor expres-sion.
