Inhibitory effects and tocolysis role of fructus polygoni orientalis on macrophages in uterine of abortion mice induced by LPS
10.3969/j.issn.1000-484X.2014.06.010
- VernacularTitle:水红花子对 LPS 诱导流产小鼠子宫巨噬细胞的抑制效应及其保胎作用
- Author:
Zhiying QIAN
;
Yajie LIU
;
Yanpin CUI
;
Lining XIE
;
Shunli ZHANG
- Publication Type:Journal Article
- Keywords:
Fructus-polygoni-orientalis;
Macrophage;
CD14+;
CD204+;
TNF-α;
Abortion;
Mouse
- From:
Chinese Journal of Immunology
2014;(6):763-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the immunosuppression effects and tocolysis of fructus polygoni orientalis on abortion mice induced by LPS.Methods:Mice of Kunming (55 mice) were divided into control group (group A ,10 mice) and experimental group. Experimental group were divided into group B ( intraperitoneal injection LPS ) , group C ( ingestion fructus polygoni orientalis ) , and group D (ingestion fructus polygoni orientalis and intraperitoneal injection LPS ),each group of 15 mice.Then the pregnancy results were observed ,the positive of α-NAE+and the varity of CD 14+and CD204+macrophages ,TNF-αin the uteri were identified by enzyme-histochemistry ,immunohistochemistry and ELISA.Results:The abortion rate and the embryo resorbing rate were all 100%( P<0.01 ) in group B.But there was the decreased abortion rate of 13.33% in group D .The embryo resorbing rate decreased to 10.39%.The number and positive cell area of α-NAE+and CD14+macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A ( P<0.01 ) .These effects outside of myometrium of group D were remarkably increased comparing with group A (P<0.01),but there was no distinct difference in the inside of myometrium and function layer.The number and positive cell area of CD204+macrophages in group C and D was greatly increased comparing with group A and B ( P<0.01 ) .The TNF-αcontents in the uteri of mice in group B were greatly increased comparing with group A (P<0.01),but the positive cell area of CD14,CD204 were close to normal levels in group D.Conclusion:The effect of miscarriage induced by LPS is antagonized by fructus polygoni orientalis through inhibiting the phenotype ,activity and function characteristics of macrophages in the uteri of gravidity mice.
