Expression and anti-tumor characterization of fusion gene GM-CSF and LMP2A of EB virus gene
10.3969/j.issn.1000-484X.2014.06.012
- VernacularTitle:GM-CSF和EB病毒LMP2 A融合基因的表达及抗肿瘤特性研究
- Author:
Qingjie XUE
;
Qiang ZHAO
;
Chuanping SI
;
Ting CHEN
- Publication Type:Journal Article
- Keywords:
Recombinant-gene-GC2A;
Anti-tumor;
Cellular-immune;
EB-virus
- From:
Chinese Journal of Immunology
2014;(6):774-778
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct GM-CSF and EB virus LMP2A fusion gene recombinant adenovirus and identificate it , using recombinant adenovirus to do immunologic study.Methods:With RT-PCR and Western blot to detect GC 2A gene expression of the recombinant adenovirus , with ELISA to analysis antibodies of vAd-GC2A stimulation in murine body , lactate dehydrogenase assay to detect the mouse cellular immunity effect , thereby the recombinant adenovirus immune effect was initially analysis.T-test method was used to do preliminary analysis and evaluation of the immune effect of recombinant adenovirus .Results:GC2A fragment amplified by PCR, the size was 1 961 bp and it was the expected.The results showed, the recombinant adenovirus vAd-GC2A proteins can be recognized by sera antibody of GM-CSF ( LMP2A); the results of ELISA showed that vAd-GC2A can stimulate the generation of antibodies.EBV-positive tumor cell killing rate (%) by CTL induced recombinant adenovirus was (66.7 ±6.9)%, at the same time the adenovirus type 5 wild strains and PBS were (24.3 ±2.5)%and(32.4 ±3.1)%only(P≤0.05).Conclusion:Fusion gene has been successfully constructed , vAd-GC2A in mice have humoral and cellular immune function.The data suggest that the vAd-GC2A should play a potent role in preventing the positive EB virus tumor.
