Serological response infected with on cynomolgus monkeys
10.3969/j.issn.1000-484X.2014.06.020
- VernacularTitle:食蟹猴实验感染HBV的血清学反应
- Author:
Zhenchang WANG
;
Zengwen LIANG
;
Jing LENG
;
Yi WEI
;
Jingjing HUANG
;
Songlin CHEN
- Publication Type:Journal Article
- Keywords:
Cynomolgus-monkey;
Hepatitis-virus;
Hepatitis-B;
Animal-model
- From:
Chinese Journal of Immunology
2014;(6):814-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the serological reactions in cynomolgus monkeys infected with hepatitis B virus ( HBV). Methods:To select 1 to 3 days old or adult healthy cynomolgus monkeys by artificial breeding to observe the virology screening in laboratory a month to confirmed healthy animals ,randomly divided into control group and infection group .Infection group vaccination serum HBV carriers 0.5 ml (HBV-DNA≥108 copies) single cages,observe each group behavioral changes daily after inoculation 1 to 12 weeks, each week to confirmed the degree of liver inflammation through the HBV-M, HBV-DNA, liver function and on the B-guided, liver tissue inflammation by routine HE staining .Results: Adult monkeys did not induce positive reaction after vaccination , there were three young monkeys appear HBsAg , HBcAb and 2 appear HBV-DNA reaction, ALT poison attack occurred in HBsAg-positive began to increase after one week , one month after the peak , which was 180 U/L, after gradually decreased , continuing a month later near normal .AST higher than a week after the normal reference values were flat curve , representing the peak ALT after a month later, HBsAg positive cynomolgus monkeys HE staining showed mild hepatitis partial liver tissue lesions .Conclusion:HBV-M, HBV-DNA, ALT, AST and liver histopathology after HBV infection have changed , this result showing that it's produce inflammation and induction the response of immune .
