MRP1 expression and bronchial epithelial function in lipopolysaccharide-induced rat model of chronic obstructive pulmonary disease
10.3969/j.issn.1005-4847.2014.03.006
- VernacularTitle:脂多糖诱导的慢性阻塞性肺病模型大鼠肺支气管上皮MRP1功能分析
- Author:
Shanshan WANG
;
Dianlei WANG
;
Xiuhua TAO
;
Chenyin WANG
;
Jinpei CHEN
;
Lili YANG
;
Yin CAO
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharide;
Chronic obstructive pulmonary disease;
Multidrug resistance-associated protein 1;
Function;
Expression;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(3):30-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of establishment of lipopolysaccharide ( LPS )-induced rat model of chronic obstructive pulmonary disease (COPD)on the function of multidrug resistance-associated protein 1(MRP1)in the rat bronchial epithelium .Methods Using intratracheal instillation of LPS to establish COPD rat model .8-week old healthy male Wistar rats were divided into 3 groups (10 rats in each group ):(1) Normal control;(2) Modeling for 14 days after LPS instillation;(3) Modeling for 28 days after LPS instillation.Pulmonary function and the concentration of phenol red in bronchoalveolar lavage fluid ( BALF) and plasma were measured .The ratio of phenol red concentration in BALF/plasma was used as an index of the MRP 1 function in the rat bronchial epithelium and the expression of MRP 1 in the bronchial epi-thelium was also observed by immunohistochemistry .Results Compared with the normal group , the pulmonary functions of the rats in the model groups were significantly reduced along with the modeling progress .After intravenous administration of phenol red, the ratio of phenol red concentration in BALF/plasma was gradually reduced , and the expression of MRP1 in the bronchial epithelium was significantly decreased .Conclusions COPD rat model can be established by intratracheal LPS instillation, and the function of MRP1 in bronchial epithelium was gradually reduced along with the modeling progress .
