ADAM8 gene promotes proliferation of colon cancer cell HCT8 through AKT/mTOR signaling pathway
- VernacularTitle:ADAM8基因调控AKT／mTOR通路对结肠癌细胞HCT8的影响
- Author:
Yang BAI
;
Hao CHEN
;
Jizheng LI
;
Jintuan HUANG
;
Zuli YANG
- Publication Type:Journal Article
- Keywords:
a disintegrin and metalloprotease domain 8;
colon carcinoma;
immunoblotting;
interference expression
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(3):24-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of a disintegrin and metalloprotease domain(ADAM8)gene on colon cancer HCT8 cell proliferation and proliferation signal transduction pathways PI3K-Akt-mTOR. Methods Colon cancer HCT8 cells were cultured in vitro,and transfected with ADAM8 overexpression plasmid and RNA interfering plasmid. Cell proliferation were detected by EdU and MTS method assays. PI3K activity was measured by PI3K activity detection kit,Western Blot method was performed to detect the ratio of Akt and p-Akt and expression of mTOR. Results Compared with control group(1.00 ±0.12),the cell proliferation in ADAM8 overexpression group(1.22 ±0.13)was significantly higher (P<0.05)and in RNA interfering group(0.78 ±0.11)was significantly lower while PI3K activity had no significant changes in three groups. After ADAM8 overexpression,and the ratio of p-Akt/Akt and mTOR expression were increased significantly,while reduced significantly after RNA interferered. Conclusion ADAM8 can promote HCT8 cell proliferation through enhancing the phosphorylation of Akt and promoting the expression of mTOR.
