Effect of daidzein on hepatocellular carcinoma SMMC7721 cell proliferation and tumor stem cell CD133 expression
- VernacularTitle:大豆黄酮对人肝癌SMMC7721细胞增殖及肿瘤干细胞CD133表达的影响
- Author:
Min ZHANG
;
Hongzhi SUN
;
Wei WANG
;
Guang BAI
;
Xiaodong YU
- Publication Type:Journal Article
- Keywords:
daidzein;
hepatocellular carcinoma SMMC7721 cell;
CD133
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(3):28-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of daidzein on hepatocellular carcinoma SMMC7721 cell proliferation CD133 expression on tumor stem cell.Methods Hepatocellular carcinoma SMMC7721 cells were cultured,digested and passaged,and divided into six groups with different drug:control group with no daidzein,100 μg/mL daidzein group,200μg/mL daidzein group,300μg/mL daidzein group,400μg/mL daidzein group ,500μg/mL daidzein.The inhibition ratio,hexokinase,alkaline phosphatase and CD133 levels in SMMC7721 cell were detected and compared at 24 h,48 h,72 h among those groups. Results The inhibition ratio was increased by Daidzein dose increasing,and decreased apparently by times extending,especially in 400μg/mL and 500μg/mL daidzeingroups.Compared with control group,the hexokinase and alkaline phosphatase activity and CD133 expression were decreased apparently in groups treated with daidzein(P<0.01).The more the dose,the higher the drop(P<0.01).Conclusion Daidzein can inhibit hepatocellular carcinoma SMMC7721 cell proliferation,and inhibit CD133 expression on tumor stem cell.
