Alteration in Leukocyte Subsets and Expressions of FcγR and Complement Receptors among Female Ragpickers in Eastern India.
10.1016/j.shaw.2016.10.004
- Author:
Nandan K MONDAL
1
;
Shabana SIDDIQUE
;
Madhuchanda BANERJEE
;
Sanghita ROYCHOUDHURY
;
Sayali MUKHERJEE
;
Mark S SLAUGHTER
;
Twisha LAHIRI
;
Manas R RAY
Author Information
1. Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata, India. nandankumar.mondal@louisville.edu
- Publication Type:Original Article
- Keywords:
complement receptors;
FcγR;
lymphocyte;
monocyte;
ragpicker
- MeSH:
Adaptive Immunity;
B-Lymphocytes;
Complement System Proteins*;
Enzyme-Linked Immunosorbent Assay;
Female*;
Flow Cytometry;
Humans;
India*;
Killer Cells, Natural;
Leukocytes*;
Lymphocyte Count;
Lymphocytes;
Memory;
Monocytes;
Neutrophils;
Occupations;
Receptors, Complement*;
Solid Waste;
T-Lymphocytes
- From:Safety and Health at Work
2017;8(2):198-205
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity. METHODS: Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of Fcγ receptor I (CD64), FcγRIII (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14. Serum total immunoglobulin-E was estimated with enzyme-linked immunosorbent assay. RESULTS: Compared with the controls, ragpickers had significantly (p < 0.0001) higher levels of CD8+T-cytotoxic, CD16+CD56+natural killer, and CD4+CD45RO+memory T-cells, but depleted levels of CD19+B-cells. The percentage of CD4+T-helper-cells was lower than the control group (p < 0.0001), but their absolute number was relatively unchanged (p = 0.42) due to 11% higher lymphocyte counts in ragpickers. In ragpickers, the percentages of CD14+CD16+intermediate and CD14dim CD16+nonclassical monocyte subsets were elevated with a decline in CD14+CD16-classical monocytes. The expressions of CD64, CD16, CD35, and CD11b/CD18 on both monocytes and neutrophils, and CD14 on monocytes were significantly higher in ragpickers. In addition, ragpickers had 2.7-times more serum immunoglobulin-E than the controls (p < 0.0001). After controlling potential confounders, the profession of ragpicking was positively associated with the changes. CONCLUSION: Ragpicking is associated with alterations in both innate (neutrophils, monocytes, and natural killer cell numbers and expression of complement and Fcγ receptors) and adaptive immunity (numbers of circulating B cells, helper, cytotoxic, and memory T cells).
