Effect of CD82 on the expression of integrin αV,β3, E-cadherin andβ-catenin in uterine epithelial cells in pregnant mice
10.3969/j.issn.1005-4847.2014.03.012
- VernacularTitle:过表达CD82对植入期小鼠子宫内膜整合素αV、β3、E-cadherin和β-catenin表达的影响
- Author:
Xiaowei WEI
;
Dongmei TAN
;
Qian ZHANG
;
Tongchuan HE
;
Yi TAN
- Publication Type:Journal Article
- Keywords:
CD82 gene;
Recombinant adenovirus;
Uterine epithelial cells;
Primary cell culture;
Pregnant mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(3):57-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective Uterine epithelial cells were isolated from pregnant mice and cultured in vitro , and exam-ined the effect of CD82 on the expression of integrin αV,β3, E-cadherin and β-catenin in the cells.Methods The uter-ine epithelial cells were primarily isolated from pregnant mouse uterus .The recombinant adenovirus containing mouse CD 82 gene which had been constructed in our lab infected the uterine epithelial cells .Immunocytochemistry was used to detect the protein expressions of integrin αV,β3, E-cadherin and β-catenin in the uterine epithelial cells , which were infected with CD82 adenovirus or not .Results 1.The purity of primary cultured cells was (93.2 ±0.6)%.2.The transfection efficiency of CD82 recombinant adenovirus was ( 92 ±4.5 )%.The adenoviral particles carrying CD 82 gene indeed ex-pressed CD82 gene and protein in the primary uterine epithelial cells after 24 hours or 48 hours.3.The uterine epithelial cells of pregnant mice on d4 expressed integrin αV, β3, E-cadherin and β-catenin.4.In contrast to the control group, when CD82 adenovirus infected cells , the uterine epithelial cells of pregnant mice on d 4 increased the expression of integrinαV,β3 and β-catenin protein, had no significant changes of E-cadherin.Conclusions CD82 may have effect on the ex-pression of integrin αV,β3 and β-catenin in mouse uterine epithelial cells before implantation .
