4-Aminopyridine (4-AP) augments Ca(2+)-dependent action potential and changes oscillatory firing patterns in rat cerebellar Purkinje cells.
10.3349/ymj.1999.40.2.112
- Author:
Wha Sook SEO
1
;
Jung Hoon SHIN
;
Chang Kook SUH
Author Information
1. Department of Nursing, Inha University College of Medicine, Inchon, Korea. wschang@dragon.inha.ac.kr
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
Purkinje cells;
oscillatory firing activity;
4-aminopyridine (4-AP);
4-AP-sensitive current
- MeSH:
4-Aminopyridine/pharmacology*;
Action Potentials/drug effects*;
Animal;
Calcium/physiology*;
Electrophysiology;
In Vitro;
Oscillometry;
Purkinje Cells/physiology*;
Purkinje Cells/drug effects*;
Rats;
Rats, Sprague-Dawley
- From:Yonsei Medical Journal
1999;40(2):112-117
- CountryRepublic of Korea
- Language:English
-
Abstract:
Intracellular recordings in cerebellar slice preparation showed that applications of 4-AP altered the pattern of oscillatory firing activity in Purkinje cells (PCs), especially yielding pronounced changes in action potential shape. 4-AP increased the amplitude and duration of action potential significantly and decreased the spike frequency. After 4-AP application, the duration of bursting was prolonged and the duration of after-burst hyperpolarization was progressively shortened. In all PCs tested, the rhythmicity of oscillatory firing activity was abolished completely at the steady state. These results suggest that 4-AP-sensitive currents determine the shape and frequency of individual Ca(2+)-dependent action potentials as well as maintaining oscillatory firing activity in PCs.
