Study on imbalance of peripheral blood Th1 7/Treg in children with chronic hepatitis B
10.3969/j.issn.1671-8348.2014.18.016
- VernacularTitle:小儿慢性乙型肝炎患者外周血Th17细胞／调节性T细胞失衡的研究
- Author:
Xiaobin CHI
- Publication Type:Journal Article
- Keywords:
T-lymphocytes,regulatory;
hepatitis-B,chronic;
T-helper-1-7
- From:
Chongqing Medicine
2014;(18):2305-2307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the frequency of peripheral blood T helper 17(Th17)and regulatory T cells(Treg)and their as-sociated cytokines level in child chronic hepatitis B(CHB)and to investigate the balance characteristics of Th1 7/Treg under differ-ent progression status in child CHB.Methods Peripheral blood and serum of 60 children with CHB and 16 healthy controls were collected for detecting the frequency of peripheral blood Fox3+Treg and Th1 7 by the flow cytometry and serum levels of Th1 7/Treg differentiation associated cytokine IL-17A,TGF-β,IL-22,IL-23,IL-6 and IL-1βwere detected by the enzyme-linked immu-nosorbent assay(ELISA).Results The frequency of peripheral blood Th1 7 and Treg,and the ratio of Th1 7/Treg in the CHB group were significantly higher than those in the healthy controls,the frequency of Th1 7 was positively correlated with disease progres-sion.In addition,serum IL-17A,IL-22 and IL-23 levels in the CHB children were higher than those in the healthy controls(P<0. 05).Conclusion Th17 and Treg play the important role in the mechanism of child CHB.The imbalance of Th17/Treg exists in child CHB.
