Effect of Buzhong Yiqi Decoction medicated serum to the expression of PI3 K、AKT、Survivin in lung adenocarcinoma cell lines A549/DDP
- VernacularTitle:补中益气汤含药血清对A549／DDP细胞PI3 K、AKT、S urvivin表达的影响
- Author:
Dan YU
;
Ning YU
;
Jiali YI
;
Ying TANG
;
Chunying LIU
- Publication Type:Journal Article
- Keywords:
A549/DDP;
Buzhong Yiqi Decoctionmedicated serum;
PI3K;
AKT;
Survivin
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(3):4-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Buzhong Yiqi Decoction medicated serum on PI3K、AKT、survivin in lung adenocarcinoma A549/DDP cells,and explore its functional mechanism.Methods Buzhong Yiqi Decoction medicated serum of middle dosage (0.576 g/mL)were prepared,and A549/DDP cells were divided into four groups:blank serum group,the best containing medicine serum group and LY294002 group (blockers group), the best containing medicine serum +LY294002 group (combination group).The protein expression of PI3K,AKT and Survivin were detected by Immunocytochemistry and Western blot.Results Compare with blank serum group,the protein expression of PI3K、AKT and Survivin in best containing medicine serum and combination group were significantly lower(P<0.05 ),and the later was more lower,but there was no significant differences between best containing medicine serum and combination group.The expression of survivin in LY294002 group were significantly lower than blank serum group(P<0.05 ).Conclusion Buzhong Yiqi Decoction can reduce the protein expression of PI3K,AKT and surviving,increase the sensitivity of A549/DDP to DDP through PI3K/AKT signal transduction pathway.
