Study on influence of AEG-1 siRNA on proliferation and apoptosis of cervical carcinoma SiHa cells
10.3969/j.issn.1671-8348.2014.17.011
- VernacularTitle:AEG-1 siRNA对宫颈癌细胞株增殖和凋亡影响的研究
- Author:
Hongwei WANG
- Publication Type:Journal Article
- Keywords:
astrocyte-elevated-gene-1;
uterine-cervical-neoplasms;
small-interfering-RNA;
apoptotic
- From:
Chongqing Medicine
2014;(17):2139-2141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of siRNA-down-regulating astrocyte elevated gene-1(AEG-1)on the proliferation and apoptosis of cervical carcinoma cells.Methods The designed and synthesized AEG-1 siRNA was transfected to the cervical car-cinoma SiHa cells.The influence of AEG-1 siRNA on the expression of AEG-1 gene was detected by real-time fluorescent quantita-tive-polymerase chain reaction (RT-PCR);the inhibiting effect of AEG-1 siRNA on the proliferation of cervical cancer SiHa cells was detected by the MTT method;the influence of AEG-1 siRNA on apoptosis and cell cycle of the cervical cancer SiHa cellswas determined by the flow cytometry.Results Compared with the control group,AEG-1 siRNA transfecting human cervical cancer Si-Ha cells could evidently down-regulate the expression of AEG-1 gene with statistical difference (P<0.01);at the same time,the dwn-regulation of AEG-1 gene expression could significantly inhibit the proliferation of the cervical cancer SiHa cells (P<0.01), promote the apoptosis of SiHa cells (P<0.01)and arrest the cell cycle in G0/G1 phase.Conclusion AEG-1 siRNA can down-regulate the expression of AEG-1 gene in the cervical carcinoma SiHa cells,inhibit the proliferation of the cervical carcinoma SiHa cells and promote their apoptosis.
