Injection of ethanol into the common bile duct to establish a rat model of biliary atresia

VernacularTitle:胆总管内注射无水乙醇致胆道闭锁动物模型的建立
Author: Juntao GE ; Long LI ; Yandong WEI ; Haibin WANG ; Guoliang QIAO ; Zhen ZHANG ; Yao LIU ; Anxiao MING
Publication Type:Journal Article
Keywords: Biliary atresia; Liver cirrhosis; Pure ethanol; Rat,model
From: Acta Laboratorium Animalis Scientia Sinica 2014;(3):50-52
CountryChina
Language:Chinese
Abstract: Objective To establish a new rat model of biliary atresia by pure ethanol injection into the common bile duct.Methods A catheter was inserted and fixed in the common bile duct in male SD rats .Saline (8 rats) or pure ethanol (16 rats) was injected through the catheter ,respectively, and the biochemical and pathological changes in the rats were examined .Results SD rats in the experimental group were divided into a persistent injury and a restoration of liver dysfunction groups according to pathological and biochemical detection .In the persistent injury group , biochemical impair-ments were significantly higher at 8 weeks after ethanol injection than those in the control group and restoration group .Dis-tinct pathological changes in the liver were observed using HE , SMA, and Masson staining .Conclusions It is a reliable animal model of biliary atresia induced by injection of pure ethanol into the common bile duct in the rat .It will provide a useful tool in future studies of biliary atresia .