EIF6 delays the kidney interstitial tissue fibrosis process by regulating the expression of TGF-β1
10.3969/j.issn.1671-8348.2014.16.018
- VernacularTitle:真核起始因子6抑制小鼠肾间质纤维化的组织学研究
- Author:
Sisi YANG
;
Jun WU
- Publication Type:Journal Article
- Keywords:
eukaryotic-initiation-factor-6;
transforming-growth-facton-β1;
renal-tubular-epithelial-cell;
kidney;
fibrosis
- From:
Chongqing Medicine
2014;(16):2022-2025
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible role of eukaryotic initiation factor 6(eIF6)in the development of kidney inter-stitial tissue fibrosis in a mouse model of unilateral ureteral obstruction(UUO).Methods (1)UUO model was set up in eIF6+/-and eIF6+/+ mice.(2)HE and Masson staining were used to histologically assess the interstitial deposition of collagen in mouse kidney.Immunohistochemistry and image analysis were applied to analyze the expression of α-SMA and TGF-β1 in mouse kidney as well as eIF6 in human kidney samples.Results 10 days after unilateral ureteral obstruction,more kidney interstitial fibrosis tissues were found in eIF6+/- mice than that in eIF6+/+ mice.TGF-β1 andα-SMA were expressed more intensive in tubular epithelial cells of eIF6+/- mice than that of eIF6+/+ mice in the UUO model.Expression of eIF6 is lower in the region of fibrosis than that in non-fibrotic area.Conclusion eIF6 might be involved in the development of kidney interstitial tissue fibrosis by regulating the expres-sion of TGF-β1 .