EIF6 delays the kidney interstitial tissue fibrosis process by regulating the expression of TGF-β1

VernacularTitle:真核起始因子6抑制小鼠肾间质纤维化的组织学研究
Author: Sisi YANG ; Jun WU
Publication Type:Journal Article
Keywords: eukaryotic-initiation-factor-6; transforming-growth-facton-β1; renal-tubular-epithelial-cell; kidney; fibrosis
From: Chongqing Medicine 2014;(16):2022-2025
CountryChina
Language:Chinese
Abstract: Objective To investigate the possible role of eukaryotic initiation factor 6(eIF6)in the development of kidney inter-stitial tissue fibrosis in a mouse model of unilateral ureteral obstruction(UUO).Methods (1)UUO model was set up in eIF6+/-and eIF6+/+ mice.(2)HE and Masson staining were used to histologically assess the interstitial deposition of collagen in mouse kidney.Immunohistochemistry and image analysis were applied to analyze the expression of α-SMA and TGF-β1 in mouse kidney as well as eIF6 in human kidney samples.Results 10 days after unilateral ureteral obstruction,more kidney interstitial fibrosis tissues were found in eIF6+/- mice than that in eIF6+/+ mice.TGF-β1 andα-SMA were expressed more intensive in tubular epithelial cells of eIF6+/- mice than that of eIF6+/+ mice in the UUO model.Expression of eIF6 is lower in the region of fibrosis than that in non-fibrotic area.Conclusion eIF6 might be involved in the development of kidney interstitial tissue fibrosis by regulating the expres-sion of TGF-β1 .