The expression of basic fibroblast growth factor and transforming growth factorβ1 in virus myocarditis in mice
10.3969/j.issn.1000-3606.2014.07.020
- VernacularTitle:碱性成纤维细胞生长因子及转化生长因子β1在病毒性心肌炎小鼠中的表达
- Author:
Tailing LU
;
Ming LU
;
Yuncui LIU
- Publication Type:Journal Article
- Keywords:
basic ifbroblast growth factor;
transforming growth factor-beta1;
viral myocarditis;
myocardial ifbrosis;
mice
- From:
Journal of Clinical Pediatrics
2014;(7):677-681
- CountryChina
- Language:Chinese
-
Abstract:
s:Objective To investigate the possible role of basic ifbroblast growth factor (bFGF) and transforming growth factorβ1 (TGF-β1) in mice with Coxsackie viral myocarditis and their relationship. Methods Eighty male BALB/c mice, 4 weeks old, were divided randomly into study group (n=40) and control group (n=40). The study group was repeated intraperitoneally injected with Coxasckie viral B3 to establish the model of viral myocarditis, while the control group was injected with virus-free Eagle’s medium in the same period. On the 7th, 14th, 28th and 42th day after the ifrst injection, 8 alive mice selected randomly from each group were sacriifced to examine the myocardial collagen volume fraction (CVF) by Masson dyeing, and to detect the protein and mRNA expression of bFGF and TGF-β1 by RT-PCR and immunohistochemistry. The correlations were analyzed. Results At each time point, the expressions of protein and mRNA of bFGF and TGF-β1 both in study group were signiifcantly higher than those in control group (P<0.01), and gradually increased over time. The expressions of protein and mRNA of bFGF and TGF-β1 were positively correlated with CVF (r=0.86~0.95, all P<0.01). In addition, the expressions of protein and mRNA of bFGF also had positive correlation with the expression of protein and mRNA of TGF-β1 (r=0.94, 0.92, P<0.01). Conclusion bFGF and TGF-β1 may promote the occurrence and development of myocardial ifbrosis in viral myocarditis, which may provide a new target for future treatment of myocardial ifbrosis.
