Biofilms produced by Haemophilus influenzae in vitro and antibiotics sensitivity changes
10.3969/j.issn.1000-3606.2014.07.021
- VernacularTitle:流感嗜血杆菌生物膜形成对抗生素的耐药性变化
- Author:
Xue GAO
;
Xiaoling SHANG
;
Haixia QIAO
;
Yanxia ZHANG
;
Cunhui ZHANG
;
Yutuo ZHANG
- Publication Type:Journal Article
- Keywords:
Haemophilus inlfuenzae;
bioiflm;
minimal biofilm eradication concentration;
antibiotic
- From:
Journal of Clinical Pediatrics
2014;(7):682-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a bioiflm (BF) models of Haemophilus inlfuenza in vitro, and to observe the changes of antibiotic susceptibility after the BF fromation. Methods Thirty strains Haemophilus inlfuenzae isolated from adenoids of children with adenoidal hypertrophy and cultured in a 96-well plate. The BF was identiifed by crystal violet staining and scanning electron microscopy (SEM). The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the minimum bioiflm bacteria bioiflm clear concentration (MBEC) of ampicillin (AMP), ceftriaxone (CRO), levolfoxacin (LVFX) and azithromycin (AZM) were individually detected. Result All of 30 strains of Haemophilus inlfuenzae formed various BF. After BF is formed, the increase of MBEC for different antibiotics was inconsistent with the increase of MIC and MBC. The difference was statistically signiifcant (MBEC/MBC, H=91.54;MBEC/MIC, H=87.91;all P<0.001). The MBEC of AMP was the highest, up to 100 times than the MBC and MIC. The MBEC of CRO was dozens of times than the MBC and MIC. The MBEC of LVFX and AZM were most close to those of MBC and MIC. Conclusion After the formation of BF, resistance to antibiotics of Haemophilus inlfuenzae is enhaced. LVFX and AZM showed more favorable effect on Haemophilus infuenzae BF.
