Behcet's disease sera containing antiendothelial cell antibodies promote adhesion of T lymphocytes to cultured human dermal microvascular endothelial cells.
10.3349/ymj.1999.40.2.152
- Author:
Kwang Hoon LEE
1
;
Hae Shin CHUNG
;
Dongsik BANG
;
Sungnack LEE
Author Information
1. Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea. kwanglee@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Antiendothelial cell antibodies;
Behcet's disease;
ICAM-1;
VCAM-1;
E-selectin;
T lymphocyte
- MeSH:
Antibodies/physiology*;
Antibodies/blood;
Behcet's Syndrome/immunology;
Behcet's Syndrome/blood*;
Blood Physiology;
Cell Adhesion/physiology;
Cells, Cultured;
Endothelium, Vascular/physiology*;
Endothelium, Vascular/immunology*;
Endothelium, Vascular/cytology;
Human;
Microcirculation/physiology;
Skin/blood supply*;
T-Lymphocytes/physiology*
- From:Yonsei Medical Journal
1999;40(2):152-158
- CountryRepublic of Korea
- Language:English
-
Abstract:
Antiendothelial cell antibodies (AECA) have been detected in the sera of patients of autoimmune diseases showing vasculitis. Using IgM-ELISA, we found AECA in 42 (56%) of 75 sera samples from patients with Behcet's disease in a previous study. All of the 15 AECA-positive sera of Behcet's disease patients had an increased expression of the intercellular cell adhesion molecule-1 (ICAM-1), 93.3% of the sera induced the vascular cell adhesion molecule-1 (VCAM-1), and 100% of the serum induced the E-selectin molecule on human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera of Behcet's disease patients, the expression of ICAM-1 and VCAM-1 on HDMEC increased significantly at 4 hours, reaching a peak at 16 hours. Expression of E-selectin was induced at 1 hour after stimulation with a peak at 4 hours and it decreased thereafter. Adherence of T lymphocytes to HDMEC increased significantly after stimulation with AECA-positive sera from Behcet's disease patients. Also, the adherence of T lymphocytes to HDMEC increased at 4 hours and returned to its normal level at 48 hours. These results show that AECA-positive sera of Behcet's disease patients are capable of activating HDMEC to promote the adherence of T lymphocytes to increase the expression of ICAM-1, VCAM-1, and E-selectin on the cell surfaces. The whole process may play an important role in the pathogenesis of vasculitis in Behcet's disease.
