Improvement effect of adrenomedullin and its binding protein-1 on intestinal mucosal barrier function in obstructive j aundice rat
10.3969/j.issn.1671-8348.2014.16.020
- VernacularTitle:肾上腺髓质素及其结合蛋白改善梗阻性黄疸大鼠肠黏膜屏障功能的作用
- Author:
Juntao YANG
- Publication Type:Journal Article
- Keywords:
adrenomedullin;
adrenomedullin-binding-protein-1;
jaundice,obstructive;
intestinal-mucosal-barrier
- From:
Chongqing Medicine
2014;(16):2028-2031
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protection effect of adrenomedullin(AM)and adrenomedullin binding protein-1 (AMBP-1) on the intestinal mucosal barrier after obstructive jaundice.Methods 24 male Sprague-Dawley rats were randomly divided into the Sham group,obstructive jaundice control group(Vehicle)and AM/AMBP-1 treatment group(n=8).Obstructive jaundice was in-duced in adult rats(180-220 g)by common bile duct ligation(CBDL).On 6 d after CBDL,the rats were administered through fem-oral vein with AM/AMBP-1(24/80μg/kg).Blood and tissue samples were collected at 24 h after administered AM/AMBP-1 for various measurements.The intestinal permeability of all rats was measured by fluorescein isothiocyanate-dextran(FITC-D).Serum concentration of TNF-αwas measured by enzyme-linked immunosorbent assay.The apoptosis of intestinal cells was detected by ter-minal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL).The expression of cleaved caspase-3 was measured by Western blot.The inj ury condition in the small intestinal mucosa was assessed by the Chiu′s scoring under microscope.Results Compared with the Sham group,the permeability of intestinal mucosa in the Vehicle group was increased (P<0.05)and serum TNF-αlevel was increased obviously (P<0.05).The expression of cleaved caspase-3 protein,the apoptosis index(AI)and the Chiu′s score were increased in the Vehicle control group(P<0.05).Compared with the Vehicle control group,the permeability of intestinal mucosa was significantly improved (P<0.05),serum TNF-αwas decreased in the AM/AMBP-1 group(P<0.05).The expression of cleaved caspase-3,the AI index and Chiu′s score were significantly decreased in the AM/AMBP-1 group(P<0.05). Conclusion AM and AMBP-1 can significantly improve the high permeability state of the intestinal mucosa in obstructive jaundice rat,reduce the apoptosis of the small intestinal tissue,alleviate the systemic inflammatory reaction and have the protective effect on the intestinal mucosal barrier.
