CD59 has a positive effect on LAT induced T lymphocytes activation
10.3969/j.issn.1000-484X.2014.07.003
- VernacularTitle:CD59促进LAT介导的T细胞活化
- Author:
Meihua GAO
;
Yuanyuan LI
;
Lina WANG
;
Beibei CONG
;
Bing WANG
;
Bei ZHANG
;
Ying LI
;
Jie LIANG
- Publication Type:Journal Article
- Keywords:
CD59;
LAT;
Jurkat;
siCD59;
Phosphorylation
- From:
Chinese Journal of Immunology
2014;(7):874-878
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the function of CD 59 in LAT induced T lymphocytes'proliferation and activation.Methods:Transfected LAT-GFP recombinant lentiviral vectors into Jurkat cells and established a fusion-protein stable express cell line ( Jurkat-GFP ).Junket-GFP cells were transfected with pSUPER-siCD59 plasmids by electroporetion or stimulated by anti-CD59 antibody.The cellular locations of CD 59 and LAT were observed under fluorescence microscope with the immunofluorescence cytochem -istry.The cells proliferation were measured by MTT assay.Furthermore,Western blot was used to detect the total and phosphorylation levels of several down-stream proteins after T cell activated .Results: Jurkat-GFP cells successfully transfected with pSUPER-siCD59 plasmids showed lower fluorescence staining.CD59 and LAT distributed uniformly on the cell surface before stimulated with anti-CD59 antibody and formed clusters once upon stimulation.Jurkat-GFP cells stimulated with anti-CD59 antibody showed a higher level of pro-liferation and protein phosphorylation ,compared with the others.Conclusion:CD59 contributed to LAT induced signaling transduction of T lymphocytes ,and stimulated CD59 molecule partly promoted T cell activation.
