Specific TCRαβgene modification promotes anti-tumor reactivity of T cells
10.3969/j.issn.1000-484X.2014.07.009
- VernacularTitle:特异性TCRαβ基因转染T细胞促进抗肿瘤免疫的研究
- Author:
Fenglin WU
;
Wenfeng ZHANG
;
Mian HE
;
Nuan YANG
;
Han SHEN
;
Huaben BO
;
Hongwei SHAO
;
Shulin HUANG
- Publication Type:Journal Article
- Keywords:
TCR;
Gene transfer;
T cells;
Anti-tumor reactivity
- From:
Chinese Journal of Immunology
2014;(7):901-908
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effects of tumor specific TCR gene Vα12.2-Vβ7.1 modification on recognition of tumor antigen and activation of anti-tumor reactivity of T cells.Methods: T cells were transduced using recombinant Ad 5F35-TRAV-TRBV adenovirus ,and multiplicity of infection was optimized.Specific lysis of T cells was evaluated by calcein release assay.The frequency of apoptotic cells in target cells was detected by Annexin V /PI double-labeled FACS.The expression of FasL on T cells was analyzed by FACS.The secretion of cytokine IFN-γand IL-2 of T cells was determined by ELISA assays.Results: The highest tranduce efficiency was obtained at MOI 100 by recombinant Ad5F35-TRAV-TRBV adenovirus.The frequency of TCRVα12+Vβ7+cells reached above 25%3 days after transduction.TCR gene modification enhanced the ability of T cells to lyse HLA-A2+AFP+target cells(P<0.001), the ability of T cells to induce HepG-2 apoptosis(P<0.001),and expression of FasL on T cells(P<0.001).TCR gene modification also enhanced T cells to secret IFN-γafter coculture with antigen positive tumor cells ( P<0.001 ).Conclusion: Specific TCR gene modification by recombinant adeno virus effectively promotes T cells to recognize antigen positive tumor cell and exert anti -tumor reac-tivity.
