Identification of IgE-reacting Clonorchis sinensis antigens.
10.3349/ymj.1999.40.2.178
- Author:
Tai Soon YONG
1
;
Soon Jung PARK
;
Du Ho LEE
;
Hye Jin YANG
;
Jongweon LEE
Author Information
1. Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea. tsyong212@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Clonorchis sinensis;
IgE;
hypersensitivity;
excretory-secretory antigens
- MeSH:
Animal;
Antibodies, Monoclonal;
Antigens, Helminth/immunology*;
Antigens, Helminth/analysis*;
Clonorchis sinensis/immunology*;
Female;
Fluorescent Antibody Technique;
Human;
IgE/immunology*;
Immunoblotting;
Mice;
Mice, Inbred BALB C
- From:Yonsei Medical Journal
1999;40(2):178-183
- CountryRepublic of Korea
- Language:English
-
Abstract:
Clonorchis sinensis is a liver fluke and it is the most prevalent human parasite in Korea at present. The parasite infection induces immune responses, characteristically an increased production of parasite-specific IgE in the host. Major IgE-reacting C. sinensis antigens in infected humans have been protein bands with MWs of 15, 28, 37, 45, 51, 56, 62, 66, 74, 97 and 160 KD identified by immunoblot analysis. Individual variations of the IgE binding pattern to C. sinensis antigens have also been documented. Using immune BALB/c mouse sera, IgE-reacting protein bands have been visualized with MWs of 28, 74, 86, 160 and several > 200 KD. One of the most strongly reacted C. sinensis antigenic proteins with a molecular weight of 28 KD was purified by gel filtration and preparative electrophoresis. Using a monoclonal antibody produced against the antigenic protein, the protein was localized in the parasite's intestine, and also found to be contained in excretory-secretory antigens.
