Expression of Cbfα1/p56 subtype in bone marrow mesenchymal stem cells from rat mandible
10.3969/j.issn.2095-4344.2014.23.003
- VernacularTitle:大鼠颌骨来源骨髓间充质干细胞中Cbfα1/p56亚型的表达
- Author:
Jianhua ZHOU
;
Yanbin XU
;
Jianzhong QIU
;
Zhenggang CHEN
;
Hongbing JIANG
;
Lili WANG
- Publication Type:Journal Article
- Keywords:
bone marrow;
mesenchymal stem cells;
ilium;
mandible;
core binding factor alpha 1 subunit
- From:
Chinese Journal of Tissue Engineering Research
2014;(23):3622-3626
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Unlike the ilium derived from the paraxial mesoderm, the mandible from cranial neural crest has a unique mechanism. Core binding factorα1 (Cbfα1) is a key transcription factor for skeletogenic process. However, the role of Cbfα1/p56 subtype in mandible tissue is yet not clear. OBJECTIVE:To research the expression of Cbfα1/p56 subtype in bone marrow mesenchymal cells from rat mandible in vitro. METHODS:Bone marrow mesenchymal stem cells from rat mandible and ilium were in vitro isolated and purified by primary culture. The characteristics of bone marrow mesenchymal cells were compared through the methods of enzyme linked immunosorbent assay and real-time PCR, including growth curve, alkaline phosphatase activity and relative mRNA expression of Cbfα1 subtypes. RESULTS AND CONCLUSION:Bone marrow mesenchymal cells from rat mandible and ilium were successful y obtained. Bone marrow mesenchymal cells from the mandible proliferated more rapidly, alkaline phosphatase activity of which was higher than iliac cells. The relative mRNA expression of Cbfα1/p56 subtype in bone marrow mesenchymal cells from the mandible was more than that in iliac cells at 6 days of culture (P<0.05), while the expression of Cbfα1/p57 in each time showed no statistical significance (P>0.05). The results showed that Cbfα1/p56 is very significant in the early osteogenic differentiation of bone marrow mesenchymal cells from the mandible.
