Bone marrow mesenchymal stem cells protect myocardial function in acute myocardial infarction through a paracrine mechanism
10.3969/j.issn.2095-4344.2014.23.008
- VernacularTitle:骨髓间充质干细胞旁分泌对急性心肌梗死心肌的保护作用
- Author:
Songping ZOU
;
Yu WANG
;
Chunyu LI
;
Xuedan FU
;
Yanli LIU
- Publication Type:Journal Article
- Keywords:
myocardium;
myocardial infarction;
mesenchymal stem cells;
mesenchymal stem celltransplantation;
intercellular signaling peptides and proteins
- From:
Chinese Journal of Tissue Engineering Research
2014;(23):3653-3659
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Adult cardiomyocytes show no regenerative ability, and celltherapy for myocardial regeneration and repair may improve myocardial ischemic injury function. OBJECTIVE:To confirm the effect and reveal the mechanism of bone marrow mesenchymal stem cells (BMSCs) on acute myocardial infarction (AMI). METHODS:BMSCs were isolated, cultured from bone marrow of Sprague-Dawley rats using density gradient centrifugation. AMI models were produced in 20 rats by ligating the left anterior descending (LAD) coronary artery, and randomly divided into model group and BMSCs group. In the BMSCs group, cells were subsequently injected with a sterile microinjection via the tail vein. RESULTS AND CONCLUSION:Six months postoperatively, the cardiac function was improved, the vessel density was increased, the percentage of apoptotic cells was decreased in the BMSCs group than that in the model group;the expression levels of inflammatory factors, including vascular endothelial growth factor, von Wil ebrand factor, transforming growth factor 3β, and interleukin-1βmRNA were significantly improved in the BMSCs group than that in the model group. These results showed that BMSCs can protect the myocardium from AMI by regulating the secretion of inflammatory cytokines and angiogenic factors.
