In vitro culture and neuron-like differentiation of human dental pulp stem cells
10.3969/j.issn.2095-4344.2014.23.019
- VernacularTitle:人牙髓干细胞的体外培养及神经样诱导分化
- Author:
Chengzhi FANG
;
Yujia YANG
;
Yue YAO
;
Xuehua HE
- Publication Type:Journal Article
- Keywords:
stem cells;
dental pulp;
celldifferentiation;
immunophenotyping
- From:
Chinese Journal of Tissue Engineering Research
2014;(23):3723-3726
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The discovery and concept of pulp tissue-derived stem cells is beneficial to the understanding of tooth development and regeneration and repair mechanisms from the cellular level. OBJECTIVE:To understand the induced differentiation capacity and induced conditions in vitro of human dental pulp stem cells into neuron-like cells. METHODS:Pulp tissue was separated from human healthy third molars. Single cellsuspensions were prepared and seeded into 6-wel plates containing alpha-modified minimum essential medium supplemented with 15%fetal bovine serum. Subconfluent cultures (first passage) of colony forming cells were induced with butylhydroxy anisole, forskolin,β-mercaptoethanol, basic fibroblast growth factor. RESULTS AND CONCLUSION:Immunofluorescence and reverse transcription-PCR assay showed that human dental pulp stem cells positively expressed stro-1, Col-I, dentin sialoprotein after 2 weeks of induction. Nestin and neuron-specific enolase were strongly expressed, but the gingival fibroblasts were negatively expressed. It indicates that adult stem cells in human dental pulp have a high neuron-like celldifferentiation potential under a certain inductive condition.
