Rabbit bone marrow mesenchymal stem cells transfected with recombinant adenovirus vectors carrying basic fibroblast growth factor:variation of cell phenotypes
10.3969/j.issn.2095-4344.2014.23.020
- VernacularTitle:碱性成纤维细胞生长因子重组腺病毒转染兔骨髓间充质干细胞的表型变化
- Author:
Taoyi CAI
;
Xiongsheng CHEN
;
Lianshun JIA
;
Yanqing SUN
;
Bin LIN
;
Changqing CHEN
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
fibroblast growth factors;
enzyme-linked immunosorbent assay;
transfection
- From:
Chinese Journal of Tissue Engineering Research
2014;(23):3727-3731
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Exogenous basic fibroblast growth factor (bFGF) plays an important role in the ligament tissue healing process, and the use of transgenic methods to transfect exogenous genes into cells can promote the secretion of bFGF. OBJECTIVE:To observe phenotypic changes and the bFGF protein expression after bFGF recombinant adenovirus was used to transfect rabbit bone marrow mesenchymal stem cells (BMSCs). METHODS:Passage 2 BMSCs were divided into three groups:Ad.bFGF-eGFP group, Ad.eGFP group and control group. Under a phase contrast microscope we observed the changes in cellmorphology. The expression of bFGF protein in BMSCs was determined by enzyme-linked immunosorbent assay (ELISA). The proliferative curve was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). RESULTS AND CONCLUSION:The transfected cells showed a uniform phenotype of fibroblasts. MTT colorimetric assay revealed that more proliferative activity of transfected BMSCs was shown in the Ad.bFGF-eGFP group than in the Ad.eGFP group and control group. ELISA results showed that expression of bFGF protein was higher in the Ad.bFGF-eGFP group than in the Ad.eGFP group and control group (P<0.05). BFGF recombinant adenovirus can induce the differentiation of BMSCs into fibroblasts, increase proliferative ability and promote the expression of bFGF protein.
