Transforming growth factor beta 3 induced odontoblast-like differentiation of stem cells from human exfoliated deciduous teeth
10.3969/j.issn.2095-4344.2014.23.023
- VernacularTitle:转化生长因子β3诱导人乳牙牙髓干细胞向成牙本质样细胞的分化
- Author:
Hui ZHOU
;
Jinmei LIN
;
Fei REN
;
Jianping LIU
;
Jincai ZHANG
;
Pingping XU
;
Qin YANG
;
Xiaochun CHEN
- Publication Type:Journal Article
- Keywords:
tooth,deciduous;
dental pulp;
stem cells;
transforming growth factor beta 3;
heparin;
dentin
- From:
Chinese Journal of Tissue Engineering Research
2014;(23):3745-3750
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have reported that the superfamily of transforming growth factors exert a role in the mineralization of various stem cells, but the combination effects of transforming growth factorβ3 and heparin on proliferation and mineralization ability of stem cells from human exfoliated deciduous teeth remains to be studied. OBJECTIVE:To explore the effect of transforming growth factorβ3 on odontoblast-like differentiation of stem cells from human exfoliated deciduous teeth. METHODS:Human deciduous teeth were col ected using enzyme digestion. The 3rd cells were incubated with 25μg/L recombinant human transforming growth factorβ3, 10 U/mL heparin or their combination. The dentin sialophosphoprotein mRNA and dentinsialoprotein expressions were detected by Q-PCR and western blot assay. Alkaline phosphatase activity was determined using alkaline phosphatase kit. RESULTS AND CONCLUSION:Stem cells from human exfoliated deciduous teeth grew wel after induction. The activity of alkaline phosphatase in the combination group was significantly higher than that in the transforming growth factorβ3, heparin and control groups (P<0.01). After combination induction, the cells were strongly positive for alizarin red staining. Results fromα-PCR and western blot assay showed that the expressions of dentin sialophosphoprotein were both remarkably increased at mRNA and protein levels. In summary, stem cells from human exfoliated deciduous teeth can differentiate into odontoblast-like cells under the induction of transforming growth factorβ3 plus heparin.
