Umbilical cord-derived mesenchymal stem cell culture:dyeing and tracer technique
10.3969/j.issn.2095-4344.2014.23.024
- VernacularTitle:脐带间充质干细胞培养中的染色标记及示踪技术
- Author:
Xia HUANG
;
Xinghua PAN
;
Rongqing PANG
;
Guangping RUAN
;
Xuemin CAI
- Publication Type:Journal Article
- Keywords:
stem cells;
mesenchymal stem cells;
umbilical cord;
cells,cultured;
stem celltransplantation
- From:
Chinese Journal of Tissue Engineering Research
2014;(23):3751-3755
- CountryChina
- Language:Chinese
-
Abstract:
The culture of umbilical cord-derived mesenchymal stem cells is extremely important for studies on umbilical cord mesenchymal stem cells. Optimization of cellculture technology is crucial for clinical application of mesenchymal stem cells and even celltherapy. Meanwhile, the labeling and tracer technique of umbilical cord-derived mesenchymal stem cells is a hotspot in stem celltransplantation. OBJECTIVE:To review the research and development of the cellmarkers and tracer methods of umbilical cord-derived mesenchymal stem cells. METHODS:A computer-based search of VIP, CNKI, Medline, Highwire and Foreign Journals Integration System databases was performed for articles concerning culture and labeling of umbilical cord-derived mesenchymal stem cells published from January 2001 to October 2013. The keywords were“stem cells, mesenchymal stem cells, umbilical cord-derived mesenchymalstem cells, cellculture, labeling methods”in Chinese and English, respectively. Final y, 35 articles were included in result analysis. RESULTS AND CONCLUSION:Umbilical cord-derived mesenchymal stem cells have not yet been widely used, mainly because of the immature isolation, culture and staining techniques of umbilical cord-derived mesenchymal stem cells. These techniques are worthy of further optimization studies. Although in recent years, cellmarkers and tracer technology of umbilical cord-derived mesenchymal stem cell s have made great progress, there are stil many problems need to be solved.
