Prokaryotic expression and preliminary identification of scavenger receptor protein
10.3969/j.issn.1673-4130.2014.12.002
- VernacularTitle:清道夫受体蛋白的原核表达及初步鉴定
- Author:
Changbing WANG
;
Jiayu ZHONG
- Publication Type:Journal Article
- Keywords:
enterovirus type 7 1;
scavenger receptors;
gene clone;
prokaryotic expression
- From:
International Journal of Laboratory Medicine
2014;(12):1523-1525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express and preliminary identify scavenger receptor B2 (SCARB2)protein.Methods SCARB2 cDNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR)from mRNA extracted from RD cells,and cloned into pMD19-T vector.Expression vector pET28a(+)/SCARB2 was constructed,and SCARB2 protein was expressed in prokaryotic ex-pression system.Obtained recombinant proteins were identified by detection of Western blot using His labeled monoclonal antibody.Results Recombinant SCARB2 proteins,expressed by induction of isopropy-β-D-thiogalactoside (IPTG),were success-fully purified and specifically recognized by His labeled monoclonal antibody.Conclusion SCARB2 proteins could be expressed and identified successfully,which could provide reference for researching mechanism of enterovirus type 71 (EV71)and scavenger re-ceptor protein,and for preparation of SCARB2 monoclonal antibodies.
