Study of RHD genes polymorphism among RhD negative Han indivduals in Xuzhou area
10.3969/j.issn.1673-4130.2014.12.021
- VernacularTitle:徐州地区汉族RhD阴性人群RHD基因多态性研究
- Author:
Zhongmei WU
;
Shuan WANG
- Publication Type:Journal Article
- Keywords:
RHD genotyping;
RhD serotyping;
polymerase chain reaction;
Jiangsu
- From:
International Journal of Laboratory Medicine
2014;(12):1571-1572
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the RHD gene polymorphism among the RhD negative Han individuals in Xuzhou.Methods The RhD antigen phenotypes were detected by routine serological techniques,and were identified with indirect antiglobulin test (IAT).The genotypes of RHD were detected by using sequence specific primer polymerase chain reaction(SSP-PCR).Results A-mong 110 RhD negative individuals,there were 5 cases carring RHD positive gene,47 cases carring RHD negative gene,22 cases carring RHD-CE(2-9)-D gene,17 cases carring DVI Ⅲ gene,2 cases carring weak D15 gene,and 17 carring DEL-1227A gene,re-spectively.Conclusion The RhD negative Han individuals present complex RHD gene polymorphism in Xuzhou region,and variant alleles such as RHD-CE(2-9)-D,DVI Ⅲ,DEL-1227A are given priority.
