Comparison of three methods for HBsAg detection
10.3969/j.issn.1673-4130.2014.12.039
- VernacularTitle:3种HBsAg检测方法的比较
- Author:
Haigang WANG
- Publication Type:Journal Article
- Keywords:
HBsAg;
colloidal gold immunochromatography assay;
ELISA;
LiCA
- From:
International Journal of Laboratory Medicine
2014;(12):1614-1615
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical applicability of Gold Lebel,ELISA and LiCA,through detecting HBsAg by all the three methods.Methods A total of 1 000 sapmles were selected,and each sample was detected HBsAg by using three methods. Each positive sampale was detected repeatedly to identify false negative and false positive.Results In all the three methods,the most sensitive and specific method was LiCA,next was ELISA,and the last was Gold Lebel.But Gold Lebel method was more sim-ple and rapid for preliminary screening for emergency or preoperative patients with its sensibility of 1 ng/mL.ELISA had too many operating steps to bring false negative and false positive,while its high sensitivity and specificity and low price might be accepted by commonage and fit for mass simples.LiCA′s especially high stability,accuracy,easily operating could better reflex HBV replication and therapy effects of antiviral drugs in spite of its slight cost.Conclusion The suitable HBsAg detection method should be chosen according to clinical demands.