Optimizing of ELISA assay for HBV serum markers
10.3969/j.issn.1673-4130.2014.12.041
- VernacularTitle:乙型肝炎病毒血清标志物ELISA法检测条件的优化
- Author:
Wenying HE
;
Kuan PENG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
enzyme linked immunosorbent assay;
critical value
- From:
International Journal of Laboratory Medicine
2014;(12):1618-1619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and approach the optimizing of ELISA assay for HBV serum markers.Methods To compare the critical value rate of flushing with distilled water,flushing with cleaning tap water and flushing without anything.Results The HBsAg critical value rates of flushing with distilled water,flushing with cleaning tap water and flushing without anything were 0.52%,2.60% and 5.56%.The HBsAb critical value rates of flushing with distilled water,flushing with cleaning tap water and flushing without anything were 5.52%,8.02% and 14.86%.The HBeAg critical value rate of flushing with distilled water,flush-ing with cleaning tap water and flushing without anything were 2.82%,2.92%and 3.55%.Conclusion Flushing with distilled wa-ter or flushing with cleaning tap water can reduce the high critical value rate because of enzyme or developer pollution.Flushing with distilled water exhibits more efficacy than flushing with cleaning tap water.
