Effects of Ginseng Aqueous Extract on Neurochemicals in Diabetic Encephalopathy Rat Brain by Online Microdialysis-LC-MS/MS
10.11895/j.issn.0253-3820.140215
- VernacularTitle:在线微透析-液相色谱-串联质谱法测定人参水提物对糖尿病脑病大鼠脑内神经活性物质的影响
- Author:
Qianqian WANG
;
Jing ZHANG
;
Zifeng PI
;
Shu LIU
;
Fengrui SONG
;
Zhiqiang LIU
- Publication Type:Journal Article
- Keywords:
Ginseng aqueous extract;
Diabetic encephalopathy;
Morris water maze;
Online microdialysis-liquid chromatography-tandem mass spectrometry;
Neurochemicals
- From:
Chinese Journal of Analytical Chemistry
2014;(7):997-1001
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the effects of ginseng aqueous extract on learning and memory abilities and neurochemicals in diabetic encephalopathy rat brain. Diabetes mellitus model was established. Cognitive abilities were evaluated with Morris water maze test by the indexes of escape latency time ( ELT) , times of passing through the target area and the percent of swimming track in goal quadrant. We applied online MD-LC-MS/MS method to determine eight neurochemicals in rat hippocampus among different groups in MRM mode. Venusil C18 column was used for separation. Treatment with ginseng aqueous extract significantly improved the cognitive abilities of diabetic rats (p<0. 05). The method had excellent linearity (R2>0. 99). The accuracy and precision could meet the analysis requirement. Comparing with the model group, the concentrations of taurine and acetylcholine were significantly increased ( p<0 . 01 ) after treated with ginseng aqueous extract, but the glutamic acid, aspartic acid,γ-aminobutyric acid, serine, dopamine and serotonine decreased dramatically. Ginseng aqueous extract can efficiently regulate the levels of eight neurochemials in diabetic rat brain to normal.
