Effects of Oxymatrine in vitro on Toll-like Receptor 9 Signal Transduction Pathway in PBMCs from Chronic Hepatitis B Patients
10.3969/j.issn.1005-5304.2014.07.009
- VernacularTitle:氧化苦参碱对慢性乙型病毒性肝炎 Toll样受体9信号通路的影响
- Author:
Ning YAO
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
toll-like receptor signal pathway;
oxymatrine;
cytokine
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(7):28-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-HBV mechanism of oxymatrine in vitro.Methods The antiviral mechanism of oxymatrine was analyzed through detecting the expression and function of TLR9 signal pathway and the secretion of antiviral cytokines from PBMCs treated by oxymatrine in vitro.Results Oxymatrine could not only induce IFN-α and IFN-γ secretions from PBMCs directly, but also effectively augmented the expressions of TLR9, MyD88 and TRAF6 at mRNA level, and activated the TLR9 signal transduction pathway. TLR9 ligand could induce IFN-α, IFN-γ and TNF-α secretion a lot from PBMCs pretreated by oxymatrine.Conclusion Oxymatrine possesses an anti-HBV activity via activation of TLR9 signal pathway and secretion of antiviral cytokines.
