Association of IL-10 Gene Polymorphism with Development of Atopy.
- Author:
Min So JUNG
1
;
Sung Sik CHOI
;
Keun Hye LEE
;
Xu Yin JI
;
Heon KIM
;
Jong Won KANG
;
Hyung Jin YOON
;
Youn Soo HAHN
Author Information
1. Department of Pediatrics, College of Medicine, Chungbuk University, Cheongju, Korea. yshahn@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
IL-10;
Gene;
Promoter polymorphism;
Atopy;
Haplotype;
Genotype
- MeSH:
Child;
Cytokines;
Eosinophils;
Genotype;
Haplotypes;
Humans;
Immunoglobulin E;
Interleukin-10*;
Linkage Disequilibrium;
Polymorphism, Single Nucleotide;
Promoter Regions, Genetic;
Risk Factors
- From:Pediatric Allergy and Respiratory Disease
2005;15(4):381-388
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: There has been accumulating evidence that interleukin-10 (IL-10) influences on the production of proinflammatory cytokines, regulating the development of atopic diseases. In this study, we tested the genetic association between IL-10 haplotype polymorphism and the development of atopy. METHODS: The frequency of three single nucleotide polymorphisms (SNPs) at positions- 1082 (A/G), -819 (C/T), -592 (A/C) and corresponding haplotypes in the promotor region of the IL-10 gene were analysed in 174 atopic and 130 non-atopic children using Taqman method. The data were assessed for correlations with the eosinophil count and total serum IgE concentration. RESULTS: Three haplotypes (ATA, ACC, GCC) were identified without any ambiguous phasing due to linkage disequilibrium among SNPs. The frequency of IL-10 haplotype ACC was higher in non-atopic children compared to atopic children. (P=0.04) The frequency of IL-10 haplotype ATA was higher in atopic children compared to non-atopic children, but a statistical significance was not found. (P=0.099) ATA/ATA and ATA/ACC accounted for 80 percent of six different genotypes. Although the frequency of ATA/ATA genotype was higher in atopic children, there was no statistical significance. Although medians of serum IgE level and total eosinophil count were higher among atopic children with ATA/ATA genotype than in atopic children with ATA/ACC, no statistical significance was found. CONCLUSION: These results suggest that IL-10 promotor polymorphism may be associated with a genetic risk factor for the development of atopy in Korean children.
