Effect of PM10 in Moxa Smoke on Apoptosis of Human Lung Adenocarcinoma Cell Line-A549 Cells
10.3969/j.issn.1005-5304.2014.07.016
- VernacularTitle:艾烟可吸入颗粒物对人肺腺癌A549细胞凋亡的影响
- Author:
Yuhai HUANG
;
Ping LIU
;
Bicheng YANG
;
Yingxue CUI
;
Chang HUANG
;
Juntian LIU
;
Baixiao ZHAO
- Publication Type:Journal Article
- Keywords:
moxibustion;
PM10 in moxa smoke;
A549 cells;
apoptosis;
Ca2+;
NF-κB p65
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(7):53-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of PM10 (inhalable particles) in moxa smoke on apoptosis of human lung adenocarcinoma cell line-A549 cells, and explore the possible mechanisms of inducing apoptosisMethods The A549 cells were studied in vitro experiment method, which were stained by Hoechest33258 staining method. Their morphological changes of apoptosis were observed by the fluorescence microscopy. The levels of intracellular Ca2+ and reactive oxygen species (ROS), and expression of NF-κB p65 were also measured.Results Some apoptotic cells were observed after treated with moxa smoke PM10 in the concerntration of 400μg/mL. After 4 hours intervention by moxa smoke PM10 in A549 cells, the intracellular Ca2+ level increased significantly (P<0.01). Compared with the blank control group, the expression of NF-κB p65 decreased significantly (P<0.05) after intervention of moxa smoke PM10 with different concerntrations for 4 hours. When the A549 cells were cultured with moxa smoke PM10 for 20 hours, the expression of p65 decreased significantly (P<0.05) in the concerntrations of 70, 280μg/mL, while there was no significant change in the concerntration of 140μg/mL (P>0.05). Compared with the blank control group, ROS level was significantly lower (P<0.05) in A549 cells after intervention of moxa smoke PM10.Conclusion PM10 in moxa smoke could induce apoptosis of A549 cells, could increase cytosolic Ca2+ level.
