Content Determination of Hypericin in Guanye Jinsitao Drop Pills by HPLC
10.3969/j.issn.1005-5304.2014.07.020
- VernacularTitle:HPLC测定贯叶金丝桃滴丸中金丝桃苷含量
- Author:
Guangshang CAO
;
Peimin YANG
;
Fang LI
- Publication Type:Journal Article
- Keywords:
Guanye Jinsitao drop pills;
Hypericin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(7):67-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To build a method for content determination of Hypericin in Guanye Jinsitao drop pills by HPLC.Methods The column was Kromasil C18 (150 mm×4.6 mm, 5μm), mobile phase was acetonitrile-0.1% phosphoric acid (16∶84), UV detection was performed at 360 nm, the flow rate was 1.0 mL/min, and the detector drift tube temperature was set at 30℃.Results The calibration curves were linear in the range of 0.132-1.188μg for Hypericin (r=0.999 9). The average recovery was 99.55% (RSD=0.78%).Conclusion The method is simple, accurate, reliable, and can be used for the quality control of Hypericin in Guanye Jinsitao drop pills.
